The Commission has been urged to do more to "break the glass ceiling" and help more women to reach the top.
The demand comes after it was revealed that the percentage of top female managers in the Commission has doubled since its President Jean-Claude Juncker took office.
Some 26 per cent of top managers are now women, up from 13 per cent in November 2014.
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Earlier this week, the figures were further bolstered with the appointments of Gertrud Ingestad as Director-General of its department for informatics (DG DIGIT) and Florika Fink-Hooijer as Director-General of its department for interpretation (DG SCIC). Maive Rute was also appointed to its joint research centre (DG JRC).
On Wednesday, Commission Vice President Kristalina Georgieva said that this is "a further step towards meeting Juncker's 40 per cent target of senior female management."
"I am happy to see that so many excellent women are working at the highest levels of the Commission, and encouraging other women to do the same."
She added that "our job on this is not yet done" and that the Commission will continue "to push for the promotion of female talent." Georgieva believes the top management of the EU's executive body should have a more balanced gender composite.
She points out that when she first presented the 40 per cent mandatory target, many male directors-general said discussions in a more gender-balanced room were "more inclusive" and "more sensitive to different points of view".
MEPs were quick to react to this week's appointments and news that the number of women in top jobs with the commission has doubled in two years.
Liberal Democrat deputy Catherine Bearder, who has campaigned on gender equality issues, said, "This is a big improvement but there's still a long way to go.
"It's up to the Commission to lead by example in breaking the glass ceiling for women in Europe."
ECR member Julie Girling said, "I am very pleased to see these appointments at a senior level. It goes without saying that they have been made on merit, some people say it must be positive discrimination but I don't agree. I meet so many talented women who just need the right environment to thrive and I'm delighted that the Commission is encouraging these conditions."
Further comment came from Italian EPP deputy Barbara Matera, who said, "I am pleased to see that the Commission is working toward the European goal of 40 per cent participation by women in executive positions.
"The new top managers appointed by the Commission is a clear proof of the excellent work that the European Parliament, together with the other EU Institutions, is doing in ensuring gender balance.
"I encourage Juncker, Georgieva, Jourova and all the college to continue this trend."
Elsewhere, Constance le Grip, EPP coordinator on the women rights and gender equality committee, said she was "pleased" with the new appointments.
She said, "This is an important step forward taken by the Commission for the access of women to positions of responsibility."
She "strongly" encourages the Council to unblock the directive on the presence of women on board of directors in firms listed on the stock market, which, she says, would allow them to "break the glass ceiling and work towards a men/women balance in decision-making entities".
Meanwhile, Malta was number one in Europe in terms of gender balance in the judiciary, says the 2016 EU justice scoreboard.
The justice scoreboard, which is published by the European Commission, compares the civil, commercial and administrative courts in Europe together in terms of statistics gathered over two years.